Forbes said Vergara's role as Gloria in Modern Family and some lucrative product endorsements helped her earn $43m (£32.6m) in the last 12 months.
It marks the fifth year the Colombian-American actress has topped the chart.
Forbes also said she earned more than any of her male counterparts in the past year.
The Big Bang Theory's Kaley Cuoco was the second-highest paid actress, earning $24.5m (£18.6m).
Cuoco tied with Vergara at the top of last year's Forbes list, when both actresses earned $28.5m (£21.6m).
The Mindy Project's Mindy Kaling is the biggest climber in this year's chart. Her earnings of $15m (£11.4m) helped her to rise from eighth place in 2015 to third this year.
Mariska Hargitay, who appears in Law & Order: Special Victims Unit, and Grey's Anatomy star Ellen Pompeo rounded off the top five.
Television's highest-paid actresses
- Sofia Vergara - $43m (£32.6m)
- Kaley Cuoco - $24.5m (£18.6m)
- Mindy Kaling - $15m (£11.4m)
- Mariska Hargitay - $14.5m (£11m)
- Ellen Pompeo - $14.5m (£11m)
- Kerry Washington - $13.5m (£10.3m)
- Stana Katic - $12m (£9.1m)
- Priyanka Chopra - $11m (£8.4m)
- Julianna Margulies - $10.5 (£7.9m)
- Julie Bowen - $10m (£7.6m).
- Source: bbc.com .
I prefer TV Fame/actors to any other when it comes to acting cos it makes more sense.Congrats Sofia